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Unraveling DNA: Molecular Biology for the Laboratory
This is the book that Philip Jardim of City College of San Francisco uses; he worked with the authors at the University of Wisconsin-La Crosse. It has very interesting experiments where the lux operon of Vibrio fisheri is cloned into an expression plasmid that's used to transform E. coli to make it bioluminescent. Recently, Fotodyne has contracted to carry the plasmids and the bacterial strains: www.fotodyne.com. I was surprised at how dim the glowing bacteria can be - it has to be really dark for you to see the bioluminescence of the E. coli transformed with the lux gene.
Reviewed by:Jim DeKloe