The results are in!

After subsequent checks at 48 and 72 hours, the broth transferred to the glass bottle, two conical tubes and the cell culture petri dish are all uncontaminated! I spent approximately 30-45 seconds doing an imaginary celebratory dance in my head before I realized it is only a matter of time before I do accidentally contaminate something. As for now though, operation Aseptic Transfer on an Open Bench is a success!

Tragically, my swab of my bench area did show one colony on the plate. One. (Boo!)

Yesterday we swabbed the... Read more

This week's focus in cell culture is on aseptic transfers because we will be getting our cells soon (Eek! I'm so excited).

On Monday we were assigned 100 mL of Luria Broth (LB in a 250 mL glass bottle) and were tasked with aseptically transferring 10 mL to 2 conical tubes, 10 mL to a glass bottle, and 5 mL to a cell culture (treated) petri dish. Here's a quick breakdown of the procedure for those not familiar: