Why are Bradford assays like Groundhog Day? You'll have to read about it to know.
Apparently I'm in movie simile mode this week too because this week was like being stuck in the movie Groundhog Day. We started our week with the Bradford assay. I made serial dilutions from our first aliquot. After checking with some other lab groups, we determined that we would have within range results using 1/10, 1/20, 1/30, 1/40, and 1/50 dilutions. Next we mapped out our 96 well plate to run our assay in duplicate. We got a beautiful standard curve with an... Read more