Chicken liver is a great learning tool! This week's adventures in Bioseparations involved homogenizing chopped liver, using it to explore the high speed centrifuge and running our old friend the Bradford Assay on it.
We started by jumping into a mixed day of centrifuge lecture and preparation of homogenization buffer. After we were sufficiently oriented with the rules for using the centrifuge and how to operate it, we were able to spin our chicken liver samples. Once we had a loose pellet, we pipetted off the supernatant, and then spun that down again. We also used different samples to... Read more