This week's focus in cell culture is on aseptic transfers because we will be getting our cells soon (Eek! I'm so excited).
On Monday we were assigned 100 mL of Luria Broth (LB in a 250 mL glass bottle) and were tasked with aseptically transferring 10 mL to 2 conical tubes, 10 mL to a glass bottle, and 5 mL to a cell culture (treated) petri dish. Here's a quick breakdown of the procedure for those not familiar:
Wearing gloves: Connect Bunsen Burner to gas and use striker to ignite. Then adjust flame for optimal height and... Read more