If it's one thing I've learned in my technique classes is that there is a lot of 'hurry up and wait' procedures. Working with proteins is definitely no exception.
For the past month or so my protein class has been working on purifying bacterial alkaline phosphatase (BAP). Rewinding back to day one of the process, we received our bacteria that had already been induced so our first step was to lyse the cells. Method used for lysing = sonication. I have to admit, this is my least favorite method for lysing cells. The sound of the sonicator... Read more