Evaluate primer sequences from the UW publication - this is similar to an activity in Dr. Porter's bioinformatics course ( Bioinformatics for Biology and Biotech : Learning guide 10. PCR and primer BLAST)
Before you begin : Go to the bottom of this page and download the worksheet. top
1. Pick a pair of primers and their corresponding probe to investigate from Table 1 below. There are 14 sets of primers (forward and reverse) and probe sets.
A. Only use the negative controls if you want... Read more